MCB 253: Experimental Techniques in Cellular Biology by Elizabeth Blinstrup Good

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Weekly Laboratory Exercises:

Week 1: Native Protein Electrophoresis

Week 2: SDS-Page

Week 3: Western Blot Detection

Week 4: Unknown Protein Indentification

Week 5: Tissue Culture

Week 6: Actin Staining

Current Events:

Current Events Presentation Information

Helpful Websites:

School of Molecular and Cellular Biology

University of Illinois, Urbana-Champaign

LON-CAPA

Instructor's Area

MCB 253 Blog

Before each lab exercise, I will post important information that you will need to know before conducting the lab exercise. These postings may include changes in protocols, location of lab equipment, and safe handling instructions for lab materials. Please check this blog before coming to class!

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Your feedback about this portfolio is important to me! Please take the time to fill out this MCB 253 Portfolio Feedback Survey. Your comments and suggestions will help me make improvments to both this portfolio and the MCB 253 course. Please be honest!

Introduction

The purpose of this portfolio is to provide both MCB undergraduates and teaching assistants with a resource that contains information about the MCB 253 laboratory exercises. Students should use this resource to prepare themselves for each lab exercise and to explore additional resources about each lab topic in order to deepen their understanding of cell biology.

Welcome to MCB 253

Welcome to MCB 253.mp3
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Course Description

Laboratory course emphasizing experimental techniques in cellular biology, cellular physiology, and developmental biology.

Credit: 2 hours

Course Information

Required Materials:

Molecular Cell Biology, Lodish, 6e

and the MCB 253 Lab Manual (can be purchased at the IUB Bookstore)

Recommended Materials:

Molecular Biology of the Cell, Alberts, 5e

Cell Biology, Pollard

Course Website:

MCB 253 Website

Instructor Contact Information

Name: Elizabeth Blinstrup

Office: 241 Davenport Hall

Office Phone: 217-244-0546

Email: blinstru@life.uiuc.edu

University of Illinois, Urbana-Champaign

Available under Creative Commons License by <a href="http://www.flickr.com/photos/jdickert/339899765/" target="_new">iLoveButter</a>

Experimental Techniques in Cellular Biology

Available under Creative Commons License by <a href="http://www.flickr.com/photos/turbojoe/1353062396/" target="_new">turbojoe</a>

MCB Discussion Groups

You may have to request membership to participate in some of these discussion groups. Please sign up for the MCB 253 Google Group. If any of the other groups interest you, you may sign-up for those also. If you find and/or belong to other MCB discussion groups not posted here, please contact the instructor with the information about the group.

Google Group- MCB 253 Students:
This group is specifically for MCB 253 students. Please request membership from the instructor.

Molecular Biology Notebook:
This group allows you to post or answer molecular biology questions.

Biochemistry and Molecular Biology:
This is a group for anyone interested/involved in the fields of Biochemistry, Genetics, Molecular Biology or other related fields.

Stem Cells:
This group is for discussion about current research with stem cells. It is NOT a group for ethical debate.